ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Objective To determine the complete genomic sequences of 3 Dengue 2 virus (DEN-2) strains isolated in Guangdong province, China and to investigate their genotypes and sources. Methods The complete genomic sequences of 3 DEN-2 strains (GD09/93, GD05/98, and GD19/2001) from Guangdong province were established by RT-PCR amplification. The phylogenetic tree of DEN-2 was constructed by Kimura method. Results The complete genomic sequences of 3 DEN-2 strains were 10 723 nucleotides (nt) in length and contained a single long open reading frame (ORF) of 10 173 nucleotides (97-10 269 nt), encoding 3391 amino acids. The ORF encoded structural and non-structural proteins, flanked by 5' and 3' non-coding regions. Comparing GD05/98 with GD09/93, GD05/98 with GD19/2001, and GD09/93 with GD19/2001, the nucleotide sequence homologies were 93.3%, 92.4%, and 97.6%, respectively, and the deduced amino acid sequence homologies were 96.7%, 96.5%, and 98.5%, respectively. Conclusion The DEN-2 strains are pathogenic in suckling mice. Compared with DEN-2-04 strain (nonpathogenic in suckling mice), there are 18 amino acid substitutions that confer changes in charge or polarity. The charge changes at PrM-134, NS2A-153, and NS4B-102 have a relatively strong impact on the antigenicity. GD05/98 strain falls within the groupⅡ comprising Thailand strains, while GD09/93 and GD19/2001 strains fall within the group Ⅳ comprising strains from Indonesia, Australian and Taiwan. There are different DEN genotypes in China, and one genotype of DEN-2 may be transmitted in different periods.
Objective To determine the population dynamics of mosquitoes and midges before and after sunset. Methods Net traps were set along certain locations at varying heights to capture the insects before and after sunset. Results Most of the captures were midges, amounting to 1039 of 7 genera, dominated by the genus Culicoides. The collected mosquitoes were of 2 species under 2 genera, totaling 53. The activity peak of the insects was approximately 1 hour after sunset. Conclusion The species composition and dynamics of mosquitoes and midges around the residential areas of Weng’ang village before and after sunset were generally clarified.
Objective This study aims to evaluate the risk for the introduction of African swine fever virus to China and to predict the potential distribution of the vectors, providing the basis for development of prevention and control strategies. Methods The adaptability analysis software, Climex, was employed to predict the potential distribution of soft ticks in China. Results The parameter setting was derived from the biological data of soft ticks and the default template parameters of Climex. The analysis revealed that the ecoclimatic indexes of soft ticks in such places as Yunnan, Guizhou, Eastern Sichuan, Chongqing, Southern Shaanxi, Hunan, Hubei, Northeastern Jiangxi, Anhui, Henan, Zhejiang, Jiangsu, Shandong and Hebei were greater than 20; these locations would be the highly suitable, potential habitats for soft ticks in China. Conclusion Some areas in China may be the natural foci of African swine fever, suggesting that the future monitoring and control should be focused on these regions.
Objective To establish a TaqMan probe?based fluorescence quantitative PCR assay for rapid detection of Dengue virus type 1 (DV1) to facilitate the clinical diagnosis. Methods A set of specific primers and TaqMan probes were designed for the RT?PCR according to the conservative gene sequences at the 5′-terminal non?coding regions of DV1. A total of 40 sera samples were collected from patients with dengue fever, and four serotypes of standard DV strains were used as the control. The specificity of the established TaqMan?based fluorescence quantitative PCR assay was determined using the RNA templates obtained through in vitro transcription in the RT?PCR of the standard strains as a positive control. The sensitivity of the assay was then compared with that of the DV?IgM/IgG?based ELISA by assessing the sera samples. Results The lowest detection limit of the established method was approximately 10 gene copies per reaction. As to the positive results among the sera samples collected from patients at different stages after onset, the RT?PCR had the highest positive detection rate during the first three days after onset (81.25%), while the ELISA?IgM had the highest positive detection rate from day 4 to day 6 after onset (85.00%). After 7 d, ELISA?IgG had the highest positive detection rate (75.00%). Conclusion The established RT?PCR assay was a highly sensitive, specific and reproducible approach for rapid detection of DV1, conducive to the early diagnosis of dengue fever.